In vitro hyperthermia based on a multi-mode detection chip with impedance sensing
XIE Xinwu1,2, LIU Weiran1, LIU Ran1
1. Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China;
2. Institute of Medical Equipment, China Academy of Military Medical Sciences, Tianjin, 300161, China
Abstract：Hyperthermia is a useful method for cancer treatment after surgery, chemotherapy and radiotherapy for numerous types of cancers. However, the mechanism is not yet fully understood and some in vitro results cannot be repeated in clinical applications; thus, further research is needed. In this study, a lab-on-a-chip device and a corresponding detection system was constructed to study in vitro hyperthermia. The device incorporated electric cell-substrate impedance sensing (ECIS), morphological imaging, and fluorescence observation during and after cells were thermally treated in situ with automatic on-chip temperature control. The temperature control was tested in an incubator. The cell culture and impedance sensing were also validated. Then, cancer cells were treated and monitored by the system for two hyperthermia conditions. The impedance curve with rectangle hyperthermia control of 41℃ for 2 h shows a "V" shape change during the 41℃ treatment with the cells quickly shrinking and re-adhering without any obvious viability changes in the fluorescent labeling images. However, two hyperthermia pulses at 55℃ for 2 min caused cell impedance "L" shape changes and killed most of the cells. These results demonstrate that this device with un-labeled and labeled methods is a useful tool for in vitro research on thermal therapy.
谢新武, 刘蔚然, 刘冉. 基于电阻抗多模式检测芯片的体外热疗[J]. 清华大学学报（自然科学版）, 2016, 56(7): 751-758.
XIE Xinwu, LIU Weiran, LIU Ran. In vitro hyperthermia based on a multi-mode detection chip with impedance sensing. Journal of Tsinghua University(Science and Technology), 2016, 56(7): 751-758.
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