Key techniques in microfluidic flow cytometers

ZHAO Jingjing; YOU Zheng

doi:10.16511/j.cnki.qhdxxb.2018.26.036

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Journal of Tsinghua University(Science and Technology) ›› 2018, Vol. 58 ›› Issue (11) : 953-960. DOI: 10.16511/j.cnki.qhdxxb.2018.26.036

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Key techniques in microfluidic flow cytometers

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Abstract

Flow cytometers are biomedical instruments for high throughput analyses and sorting of single cells which are widely used in both biomedical research and clinical diagnoses. Advances in microfluidics have led to highly-integrated, compact, fully-enclosed, and no cross-contamination microfluidic flow cytometers. This study focuses on three key techniques with 3-D focusing of the sample flow, laser beam shaping, and on-chip sorting. 3-D focusing microfluidic chips confine the sample flow down to 10 micrometers at a velocity of several meters per second. Specially-designed binary optical elements (BOEs) generate micrometer-scale rectangular quasi-flat spots for exciting fluorescence that replace conventional elliptical Gaussian spots. These give an on-chip sorting mechanism based on the jet-flow by expanding spark-induced cavitation microbubbles. A microfluidic test system is developed by combining these three techniques with performance that is close to that of two commercial instruments.

Key words

flow cytometer / microfluidics / cell sorting / binary optical element / fluorescence detection / flow cytometry

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ZHAO Jingjing, YOU Zheng. Key techniques in microfluidic flow cytometers[J]. Journal of Tsinghua University(Science and Technology). 2018, 58(11): 953-960 https://doi.org/10.16511/j.cnki.qhdxxb.2018.26.036

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