Autofluorescence of chloroplasts measured by a laser scanning confocal microscope

FENG Qianqian; WANG Wenjuan; LI Haidong; PAN Xun

doi:10.16511/j.cnki.qhdxxb.2017.26.034

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Journal of Tsinghua University(Science and Technology) ›› 2017, Vol. 57 ›› Issue (6) : 651-654,660. DOI: 10.16511/j.cnki.qhdxxb.2017.26.034

BIOLOGICAL SCIENCE AND ENGINEERING

Autofluorescence of chloroplasts measured by a laser scanning confocal microscope

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Abstract

The autofluorescence of nicotiana benthamiana chloroplasts was studied using a laser scanning confocal microscope. The chloroplast autofluorescence was obtained by spectral scanning with various excitation wavelengths. Then, chloroplast autofluorescence images were obtained using laser scanning confocal microscopy with various excitation wavelengths with intensity quantification. Finally, nicotiana benthamiana chloroplasts were illuminated with 488 nm light to test the autofluorescence stability. 488 nm light has the highest excitation efficiency for chloroplast autofluorescence, while 561 nm light has the lowest excitation efficiency. The autofluorescence chloroplast emission spectrum starts from 637 nm and peaks at 681 nm. The measurements show that the chloroplast autofluorescence is very stable and that laser scanning confocal microscopy can accurately measure chloroplasts autofluorescence. This work will facilitate improved plant tissue imaging chloroplast genetic engineering research.

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laser scanning confocal microscopy / chloroplast / autofluorescence / spectral imaging

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FENG Qianqian, WANG Wenjuan, LI Haidong, PAN Xun. Autofluorescence of chloroplasts measured by a laser scanning confocal microscope[J]. Journal of Tsinghua University(Science and Technology). 2017, 57(6): 651-654,660 https://doi.org/10.16511/j.cnki.qhdxxb.2017.26.034

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